Fig S1 Species phylogeny based on single copy orthologs_protein sequence alignment

The concatenated protein sequence alignments of nine anopheline and two outgroup dipteran species (created using MUSCLE and trimmed with trimAl) of 4,829 relaxed single-copy orthologs (a maximum of three paralogs allowed in no more than two species, longest protein selected) determined by OrthoDB orthology delineation (available at http://cegg.unige.ch/orthodbmoz2), which resulted in 3,400,861 amino acid columns (with 906,126 distinct alignment patterns)