DZNep destabilizes cFLIP mRNA and accelerates cFLIP protein degradation.

<p>(<b>A</b>) cFLIP and A20 mRNA expression in DZNep-treated cells (1 μM, 24 h) analyzed by qRT-PCR. Data are shown as mRNA expression values relative to a control. (<b>B</b>) Analysis of cFLIP mRNA expression determined by qRT-PCR of cells treated with DZNep (1 μM) for 24 h followed by incubation with actinomycin D (2.5 μM, 2–6 h). (<b>C, D</b>) Expression level of cFLIP analyzed by Western blotting of cells treated with DZNep (1 μM) for 24 h followed by incubation with cycloheximide (50 μM, 0.5–6 h). Quantification of cFLIP expression by densitometry using ImageJ. (<b>E</b>) Relative expression levels of miRNA-512–3p in cells incubated with DZNep (1 μM) for 8 or 24 h analyzed by qRT-PCR. (<b>F</b>) cFLIP expression levels in DZNep-treated (1 μM, 24 h) BJAB cells after transfection with anti-miR-512–3p and anti-miR-346. Protein transfer was confirmed by ponceau S staining, and equal loading was confirmed by total β-actin.