Six channel FDMM for subcellular staining of cultured NIH cells.
- Siavash Kijani (698407)
- Ulf Yrlid (698408)
- Maria Heyden (698409)
- Malin Levin (396280)
- Jan Borén (119843)
- Per Fogelstrand (396278)
Publication date
March 2015
DOIAbstract
<p>Cultured NIH cells immunolabeled for different intracellular structures. Upper small pictures: Nuclei (DAPI channel, DAPI, 12 ms), Focal adhesions (425 channel, anti-vinculin, 90 ms), Lipid droplets (488 channel, anti-ADRP, 50 ms), Golgi (Cy3 channel, anti-syntaxin 5, 10 ms), F-actin cytoskeleton (594 channel, phalloidin, 17 ms), and Lyzosomes (PerCP channel, anti-LAMP 1, 46 ms). Scale bar, 40 μm. Objective x63/1.4 Oil DIC. Lower large picture: Merged image of all channels. Scale bar, 20 μm.</p
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