Detection of lipid-loaded dendritic cells in an atherosclerotic lesion using FDMM.

<p>Atherosclerotic lesions were induced in the carotid artery of LDLr^−/− mice. The carotid arteries were harvested, sectioned and multi-immunolabeled with antibodies against lipid droplets (anti-perilipin 2, red), smooth muscle cells (anti-SM α-actin, cyan), antigen presenting cells (anti-MHC class II, green, upper left), CD11c (anti-CD11c, green, lower left), and CD11b (anti-CD11b, green, lower right). Nuclei were stained with DAPI (blue). Arrows depict cells that are positive for MHC class II, CD11c and perilipin 2, and negative/dim for CD11b. The small arrowheads indicate arterial elastin fibers (laminae), which autofluoresce in the 425 channel. All six channels are presented individually in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119499#pone.0119499.s005" target="_blank">S5 Fig</a>. Scale bar, 100 μm.