AMPK acts downstream SIRT1 in <i>Leishmania</i>-infected macrophages.

<p>Infected BMMo from WT and SIRT1 KO were treated with AICAR or simultaneously with AICAR and compound c. (A) At 14 hour post-infection the protein levels of AMPK-P-Thr172 and AMPK were determined. (B) At 18 hour post-infection the protein levels of PGC-1α were determined. Actin was used as loading control. Protein quantification is expressed as folds in comparison to uninfected BMMo after normalization with actin. (C) The levels of <i>Slc2a1</i>, <i>Slc2a4</i> and <i>Ppargc1a</i> transcripts from WT and SIRT1 KO infected and/or treated cells were analyzed by qPCR. (D) Whole cell extracts of macrophages recovered from the spleen of uninfected (Un) or 18-hour infected Mac-SIRT1 KO mice (Inf) were probed with specific antibodies for AMPK-P-Thr172 and AMPK. Actin was used as loading control. (E) Graphic represents the corresponding densitometry analysis. Means ± SD are from two representative mice from two independent experiments. *p <0.05, **p <0.01, ***p <0.001; Significant differences between WT and Mac-SIRT1KO splenic macrophages. ^#p <0.05, ^##p <0.01.