Effect of IFITM1 on HIV-1_BH10 and HIV-1_NL4–3 in the one-round infection assay.

<p>SupT1 cells were treated with doxycycline (500 μg/ml) for 16 hours to induce IFITM1 expression before infection with HIV-1_BH10 (A, B) or HIV-1_NL4–3 (C, D). Forty hours after infection, cells were harvested and stained with FITC-conjugated anti-p24 antibody. Infected cells were scored by flow cytometry (shown in A and C). Amounts of viruses made in the supernatants were determined by ELISA to measure HIV-1 p24/CA (shown in C and D). Results of three independent experiments are summarized in the bar graph.