Flow cytometry and immunofluorescence analyses of EGFR distribution.

<p>(A, B) Cells not treated with 0.05% Triton X-100 before antibody staining, (C, D) Cells permeabilized by 0.05% Triton X-100 before antibody staining. Isotype rabbit immunoglobulin G (IgG) was used as the negative control. (E) Cells were stained for EGFR with Alexa 488-conjugated EGFR antibody and visualized under a fluorescence microscope at 488 nm excitation light. Scale bar is 10 μm.