Expression profiles of <i>BmANTI1</i> and <i>BmANTI2</i> genes.

<p>The developmental and tissue-specific expression of <i>BmANT</i> genes. (A) Developmental expression profiles of <i>BmANTI1</i> and <i>BmANTI2</i> genes. Whole body RNA samples were extracted from embryo to adult, and subjected to semi-quantitative reverse transcription (semi-qRT)-PCR analysis. Total RNA from BmN4-SID1 cells was also included. Amplifications of GAPDH cDNA were used as an internal control. Individuals from 4th instar larvae to adult were divided into female and male. (B) Tissue expression profiles of <i>BmANTI1</i> and <i>BmANTI2</i> genes. The fat body (FB), gut (GU), testis (TE), Malpighian tubules (MT), and silk gland (SG) were retrieved from individuals at day 5 of 5th instar male larvae, and their total RNAs were subjected to semi-qRT-PCR analysis. (C) Testis expression profiles of <i>BmANTI1</i> and <i>BmANTI2</i> genes at the 4th and 5th instar larvae. The expression levels of <i>BmANT</i>s in the testis were measured by real-time PCR. Silkworms started to spin silk between day 7 and 8 of the 5th instar larvae. Relative expression levels against the <i>Bmrp49</i> gene in the testis are shown. Error bars represent the SD values of the means of triplicates.