Mn-L1 and Mn-L2-inhibited LPS-induced pro-inflammatory gene expression in THP1 human macrophages.

<p><b>(A) Inhibition of pro-inflammatory gene expression in macrophages by Mn-L1 and Mn-L2</b>. THP-1 macrophages were treated for 3 hours with 25 μmol/L of Mn-L1 or Mn-L2, the medium was removed and the cells were incubated one hour with fresh medium containing 100 ng/ml LPS. The mRNA levels were determined by quantitative real time PCR. The values are represented as percentages of the only LPS-stimulated control group. LPS 100% values (mean ± SE) were 5.5 ± 0.9; 63.58 ± 3.7; 74.12 ± 4.4; 13.83 ± 7.7.42 ± 5; and 13.15 ± 4 for GRP78, TNF-α, IL-6, Il-1ß, IL-8 and PTGS2 respectively. <b>(B) Inhibition of the production of pro-inflamatory TNF-α and IL-6 in macrophages by Mn-L1 and Mn-L2</b>. THP-1 macrophages were treated for 3 hours as explained in the figure. After 16 hours’ incubation with 500 ng/ml LPS, TNF-α, and IL-6 protein expression was determined in the cell culture medium. The data of three separate experiments performed in duplicate are shown. * P < 0.01 compared with LPS-treated group.