S6KL functions presynaptically in regulating NMJ growth.

<p>(A–E) Representative NMJ4 synapses from different genotypes doubly stained with anti-HRP (green) and anti-CSP (magenta). (A) wild type, (B) <i>elav-Gal4/+; UAS-S6KL/+</i>, (C) <i>C57-Gal4/UAS-S6KL</i>, (D) <i>elav-Gal4/+; S6KL RNAi/+</i>, and (E) <i>S6KL RNAi/+; C57-Gal4/+</i>. Scale bar, 5 μm. (F, G) Statistical results of the number of boutons (F) and satellite boutons (G) in different genotypes. <i>n</i> >16 for each genotype; ***<i>p</i> < 0.001 by one-way ANOVA with Tukey post hoc test; error bars indicate SEM.