Phosphoproteome survey of Kc cells under <i>Pvr</i> rescue conditions.

<p>(A) From left to right the conditions for Kc cells subjected to phosphopeptide enrichment and mass spectrometry were ‘high PVR, low InR’, ‘high PVR, low EcR, and ‘high PVR, high InR’. Of 3380 unique phosphosites identified by mass spectrometry in both replicates, 144 phosphosites were upregulated on average >1.5-fold (red) in response to insulin while 176 phosphosites were downregulated on average >1.5-fold (green). Knockdown of <i>EcR</i> induced more than 1.5-fold up- and down-regulation of 6 and 2 phosphosites, respectively. (B) From left to right the conditions for Kc cells subjected to phosphopeptide enrichment and mass spectrometry were ‘low PVR, low InR’, ‘low PVR, low EcR, and ‘low PVR, high InR’. Of 2397 unique phosphosites identified in both replicates, 237 phosphosites were upregulated on average >1.5-fold in response to insulin while 222 phosphosites were downregulated on average >1.5-fold in Pvr RNAi cells. <i>EcR</i> knockdown induced more than 1.5-fold up- and down-regulation of 8 and 9 phosphosites, respectively in PVR RNAi cells.