Effect of dietary iron loading on the expression of nicotinic acetylcholine receptors in young rat brain.

<p>Prefrontal cortex (<b>A</b>) and hippocampus (<b>B</b>) were collected from rats fed iron-loading or control diet and homogenized for western blot analysis to determine the expression levels of α7-nicotinic acetylcholine receptor. Relative intensities of protein bands normalized to actin were determined using Image Lab. Empty and closed bars represent control (Con) and iron-loaded (IL) rats, respectively. Data were presented as means ± SEM (n = 4 per group) and were analyzed using two-sample <i>t</i>-test. * <i>P</i> < 0.05.