Cleavage of alpha-synuclein occurs in Mn-treated slices.

<p>After brain slices were treated with calpain inhibitor II and Mn, protein expression of full-length, C- and N-terminal fragments of alpha-synuclein were measured from brain homogenates. (A) Western blotting for full-length, C- and N-terminal fragments of alpha-synuclein and β-actin in the calpain inhibitor II and Mn-treated slices. (B) Semi-quantitative analysis of the protein expression of full-length alpha-synuclein. (C) Semi-quantitative analysis of the protein expression of C-terminal fragments of alpha-synuclein (C-terminal alpha-synuclein CCP Ab). (D) Semi-quantitative analysis of the protein expression of N-terminal fragments of alpha-synuclein (N-terminal alpha-synuclein CCP Ab). Expression of full-length, C- and N-terminal fragments of alpha-synuclein protein was normalized with β-actin protein. ** <i>P</i><0.01 compared with control slices; ^#<i>P</i><0.05 and ^{# #}<i>P</i><0.01, compared with 400 μM Mn-treated slices.