Expression of CD27 on lymphocytes isolated from various tissues of rhesus macaques detected by flow cytometry.

<p>(A) Representative plots generated from whole blood samples showing gating strategy for analysis of CD27+ lymphocytes isolated from all tissues. Specifically, singlets were gated first followed by lymphocytes, and then live lymphocytes (DLneg) were selected for further analysis of CD3+, CD20+, CD27+ as shown as in A; (B) Comparison of percentages of various cells including CD3+, CD20+ and CD27+ after different processing of the same tissues and gating through live lymphocytes. Note that significantly decreased CD27 expression is only detected on lymphocytes subjected to collagenase digestion procedures when compared to no digestion. In contrast, there are no changes in expression of CD3 or CD20 on lymphocytes indicating this is selective for CD27. Bars represent mean percentages ± SEM in each group (n = 7), P values ≤ 0.05 were considered statistically significant.