Immunoblot detection of RAGE.

<p>QUIN-induced intracellular RAGE expression was suppressed by the antioxidant S-allylcysteine (SAC). In <b>A</b>, <b>B</b> and <b>C</b>, RAGE expression at 30, 60 and 120 min post-lesion in whole striatal extracts (35 μg protein/lane), respectively, is shown. Bands correspond to full-length (FL-RAGE) form of the protein (found around 50 kDa). Results are expressed as fold change compared with Sham. Each image represents three independent experiments. In <b>D</b>, the plot shows relative RAGE expression normalized to actin. Significant differences against Sham (*P<0.05 and **P<0.01) or QUIN (^#P<0.05 and ^##P<0.01) were considered. One-way ANOVA followed by Tukey’s test for multiple comparisons was used.