Actin polymerization regulates E-cadherin expression and RhoA GTP levels in MCF-7 cells.

<p>MCF-7 cells were treated with 100 μM Cyt D for 12 h (A) or 0–400 nM Jasplakinolide (JP) for 12 h. (B) and lysates were blotted for E-cadherin and β-actin. (C) RhoA GTP and total RhoA level were determined in MCF-7 cells treated with 100 μM Cyt D for 12 h. ***<i>p</i>>0.001; relative to control. (D) MCF-7 cells were transfected with dominant-negative (DN), wild type (WT) and dominant-active (DA) RhoA for 48 h after which cell lysates were probed for c-Myc, E-cadherin and β-actin. (E) Untransfected MCF-7 cells or MCF-7 cells transfected with dominant-active RhoA active were treated with 100 μM Cyt D for 12 h and cell lysates probed for c-Myc, E-cadherin and β-actin.