The F12/KLC2 interaction requires virus infection.

<p>SDS-PAGE and immunoblot analysis of reciprocal F12-HA and Flag-KLC co-immunoprecipitations in the presence and absence of infection. Clarified cell lysates were generated from HEK TRex-F12-HAco cells transfected with either pFlag-KLC1, pFlag-KLC2 or pFlag-GFP and infected 24 h later with vΔF12 or mock-infected for 14 h as indicated. F12-HA expression was induced by addition of doxycycline for all samples in (A) and as indicated (dox +) in (B). Cell lysates were subjected to either anti-Flag IP (A) or anti-HA IP (B) and the levels of co-immunoprecipitated proteins were analysed as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004723#ppat.1004723.g001" target="_blank">Fig. 1</a>. The positions of molecular mass standards (kDa) are shown on the left.