Terminal Complement Complex (TCC) generation induced by the <i>Premolis semirufa</i>’<i>s</i> bristles extract.

<p>Samples of NHS (50 μL) were incubated with the bristles extract (175 μg/mL) or PBS, in the presence or absence of 10 mM 1,10-Phenanthroline (Phen), at 37°C for 30 min, and SC5b-9 complex present in human serum samples was measured using the MicroVue SC5b-9 Plus EIA Kit. Data are representative for two separate experiments, performed in duplicate, and the results are expressed as concentration of SC5b-9 complex per mL of human serum (ng/mL) ± SD. (***) <i>p</i> < 0.001: significant differences between the mean values obtained with the buffer and between the treatments.