Anaphylatoxins generation from purified human C5 induced by the <i>Premolis semirufa’s</i> bristles extract and Ps82.

<p>Samples of 3 μg of the extract or 0.1 μg of Ps82 were incubated, in the absence or presence of 10 mM PMSF, with human C5 (3 μg) for 30 min at 37°C. The generation of the anaphylatoxin C5a was measured using the "Human C5a ELISA Kit". Data are representative for two separate experiments, performed in duplicate, and the results are expressed as concentration of anaphylatoxin per mL (ng/mL) ± SD. (**) <i>p</i> < 0.01 and (***) <i>p</i> < 0.001: significant differences between the mean values obtained with the Buffer and the treatments.