Inhibition of endogenous HPV16 <i>E6/E7</i> expression: Effects on selected intracellular miRNAs.

<p><b>(A)</b> Immunoblot analysis of HPV16 E7, HPV16 E6, p53 and p21 protein levels, 72 h after transfection of SiHa cells with si16E6/E7 or control siRNA (siContr-1), or upon mock treatment. α-Tubulin: loading control. <b>(B)</b> qRT-PCR analyses of ten selected cellular miRNAs, 72 h after transfection of SiHa cells with si16E6/E7 or siContr-1. Cellular miRNA levels were normalized to the snRNA <i>RNU6–2</i> and calculated relative to siContr-1 (log₂ display). Dashed lines: 1.5-fold up- or downregulation (log₂(1.5) = 0.585). Data represent mean ± SEM (n = 3). Asterisks indicate statistically significant differences (p ≤ 0.05 (*) and p ≤ 0.01 (**)).