Nutritional perturbation in mice.

<p><b>(A</b>) Body weights of male C57Bl/6J mice for 12 weeks on either control diet (CD), high-fat diet (HFD), or ketogenic diet (KD) n = 15–30. (<b>B</b>) Serum non-esterified free fatty acids (NEFA), (<b>C</b>) beta-hydroxybutyrate (β-OH), and (<b>D</b>) blood glucose in CD, HFD, KD, Fasted (Fast), Fasting-Refed (FR), and cold exposed mice, n = 8–10. <b>(E</b>) Whole body, <b>(F</b>) liver, (<b>G</b>) gonadal white adipose, and (<b>H</b>) inguinal white adipose weight in CD, HFD, KD, Fast, FR, and cold exposed mice, n = 10–15. Data represent mean ± SEM, * represents p≤0.05 by Student’s t-test relative to the CD group. Significant differences among group means are represented by letters and were determined by Tukey multiple comparison tests (p<0.05) after one-way ANOVA.