Limit of detection of designed PCR in amplifying the S gene of HBV DNA.

<p>The PCR was performed on serially diluted World Health Organization International Standard for Hepatitis B virus DNA with an initial viral concentration of 1x10⁶ IU/mL. Lane 1: 1000bp ladder; lane 2: DNA from HBV infected sample (positive control); lane 3: 20,000IU/mL, lane 4: 2,000IU/mL; lane 5: 200IU/mL, lane 6: 100IU/mL; lane 7: 50IU/mL; lane 8: 25IU/mL; lane 9: 12.5IU/mL; lane 10: 6.25 IU/mL; lane 11: 3.125IU/mL; lane 12: 1.5625IU/mL; lane 13: dH₂O (negative control).