Type-2 Helper T Cells Accumulate in the Lungs of Mice Infected with <i>C. neoformans</i>.

<p>(<b>A</b>) Cda2-MHCII tetramer identifies <i>C. neoformans</i>-specific helper T (Th) cells from mice 14 days post-infection with strain KN99α, <i>cda2Δ</i>, or age-matched, naïve mice. Flow cytometry plot (<b>B</b>) or graphs (<b>C&D</b>) from lung digests showing CD4+, Foxp3-, CD44+ Cda2+ Th cells expressing Th1 (IFNγ), Th2 (IL-5 & IL-13), or Th17 (IL-17A) cytokines. (<b>E</b>) Cytokines from lung homogenates 14 days post-infection with KN99α or age-matched, naïve mice. Flow cytometry plot (<b>F</b>) and graphs (<b>G&H</b>) from mediastinal lymph node suspensions of Th cells expressing Th1, Th2, or Th17 cytokines. Data are presented as mean +/- standard error with 2 independent experiments of at least 5 mice per group. Cda = Chitin deacetylase, CCL = chemokine ligand, IFN = interferon, IL = interleukin, MLN = mediastinal lymph node, TNF = tumor necrosis factor.