<p>(<b>A</b>) Schematic representation of <i>cap350</i> exons 5–7 indicating the E5-I5 junction targeted by the morpholino Mcap350 (red arrowhead). Exon and intron sizes as well as intron retention and cryptic donor and acceptor sites are indicated by red dotted lines. (<b>B</b>) RT-PCRs show <i>cap350</i> aberrant splicing products in morpholino-injected embryos as compared with normal splicing (350 bp band) in wild-type and mock-injected embryos. PCR products derived from intron 5 retention (I5_Reten) and cryptic donors at exon 5 (E5_C.donor) and intron 5 (I5_C.donor) are indicated (red arrows). (<b>C–D</b>) Lateral views showing the progression of the epiboly front (arrow heads and dotted line) in Mock (<b>C</b>, <b>C'</b>) and Mcap350 (...
<p><b>A</b>. LCN7 expression was detected in developing zebrafish embryos by RT-PCR analysis. cDNAs ...
<p>(<b>A</b>) Splicing MO targeting <i>dkc1</i> sequence caused the inclusion of intron 4 into mRNA ...
<p>(A) Splice targeting morpholinos were designed against intron-exon boundaries within the <i>dnm2<...
<p><b>A, B, G, H:</b>MO1 C (A, B) and MO C (G, H) embryos at 72 hpf. <b>C, D, I, J:</b> MO1 (C, D) a...
In this video, we demonstrate the technique of microinjection into one-cell stage medaka embryos. Me...
<p>(A, B) <i>In vi</i>vo imaging of an siRNA-injected embryo (200 µM Stealth-RNA, 1-cell stage micro...
<p>(A) Diagram of splicing junction morpholino targeted against <i>Mtmr8</i> exon-intron boundary. (...
<p>A. B. Schematic representation shows the genomic organization of the <i>lin-28a</i> and <i>lin-28...
<p>DIC imaged larvae developed from morpholino-injected eggs after 24hpf and 48hpf development or at...
<p>A. RT-PCR detection of PIP5Kαb and eIF1α in wild-type and <i>ocrl<sup>-/-</sup></i> embryos at th...
(A) Medaka zygotes were microinjected with Cas9 mRNA and an injection mix according to Table 1. The ...
Medaka is a small egg-laying freshwater fish that allows both genetic and embryological analyses and...
e1937, doi:10.3791/1937 (2010). In this video, we demonstrate the technique of microinjection into o...
(A to R) Medaka Ol-bsf expression investigated by fluorescence using a transgenic reporter line for ...
<p><b>Copyright information:</b></p><p>Taken from "Inhibition of primordial germ cell proliferation ...
<p><b>A</b>. LCN7 expression was detected in developing zebrafish embryos by RT-PCR analysis. cDNAs ...
<p>(<b>A</b>) Splicing MO targeting <i>dkc1</i> sequence caused the inclusion of intron 4 into mRNA ...
<p>(A) Splice targeting morpholinos were designed against intron-exon boundaries within the <i>dnm2<...
<p><b>A, B, G, H:</b>MO1 C (A, B) and MO C (G, H) embryos at 72 hpf. <b>C, D, I, J:</b> MO1 (C, D) a...
In this video, we demonstrate the technique of microinjection into one-cell stage medaka embryos. Me...
<p>(A, B) <i>In vi</i>vo imaging of an siRNA-injected embryo (200 µM Stealth-RNA, 1-cell stage micro...
<p>(A) Diagram of splicing junction morpholino targeted against <i>Mtmr8</i> exon-intron boundary. (...
<p>A. B. Schematic representation shows the genomic organization of the <i>lin-28a</i> and <i>lin-28...
<p>DIC imaged larvae developed from morpholino-injected eggs after 24hpf and 48hpf development or at...
<p>A. RT-PCR detection of PIP5Kαb and eIF1α in wild-type and <i>ocrl<sup>-/-</sup></i> embryos at th...
(A) Medaka zygotes were microinjected with Cas9 mRNA and an injection mix according to Table 1. The ...
Medaka is a small egg-laying freshwater fish that allows both genetic and embryological analyses and...
e1937, doi:10.3791/1937 (2010). In this video, we demonstrate the technique of microinjection into o...
(A to R) Medaka Ol-bsf expression investigated by fluorescence using a transgenic reporter line for ...
<p><b>Copyright information:</b></p><p>Taken from "Inhibition of primordial germ cell proliferation ...
<p><b>A</b>. LCN7 expression was detected in developing zebrafish embryos by RT-PCR analysis. cDNAs ...
<p>(<b>A</b>) Splicing MO targeting <i>dkc1</i> sequence caused the inclusion of intron 4 into mRNA ...
<p>(A) Splice targeting morpholinos were designed against intron-exon boundaries within the <i>dnm2<...