PRICKLE interacts with USP9X via its carboxyl terminal.
- Lily Paemka (492129)
- Vinit B. Mahajan (119189)
- Salleh N. Ehaideb (492131)
- Jessica M. Skeie (129390)
- Men Chee Tan (701345)
- Shu Wu (492135)
- Allison J. Cox (701346)
- Levi P. Sowers (492130)
- Jozef Gecz (9483)
- Lachlan Jolly (701347)
- Polly J. Ferguson (434720)
- Benjamin Darbro (489167)
- Amy Schneider (556109)
- Ingrid E. Scheffer (701348)
- Gemma L. Carvill (492143)
- Heather C. Mefford (364655)
- Hatem El-Shanti (492142)
- Stephen A. Wood (215371)
- J. Robert Manak (138266)
- Alexander G. Bassuk (66380)
Publication date
March 2015
DOIAbstract
<p> <b>A.</b> PRICKLE1 and PRICKLE2 interact with endogenous Bcr, Tanc2, and Usp9x in NGF-differentiated PC12 cells. GFP immunoprecipitates from stable lines expressing GFP, GFP-PRICKLE1 or GFP-PRICKLE2 confirm that PRICKLE interacts with Bcr, Usp9x and Tanc2. <b>B</b>. Flag immunoprecipitates, from HEK293T cells overexpressing flag-tagged PRICKLE1 or PRICKLE2, show endogenous USP9X physically interacts with PRICKLE. <b>C, D.</b> Schematic of PRICKLE1(C) and PRICKLE2(D) constructs. Flag-immunoprecipitates from HEK293T cells overexpressing the indicated constructs were analyzed by anti-USP9X Western blotting. Both PRICKLE 1 and 2 interact with USP9X via their C-termini while BCR binding mapped to their N-termini.</p
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