Virus infection induces TRIM26 expression and nuclear translocation.

<p>(A) Western blot analysis of TRIM26 protein expression in mouse primary peritoneal macrophages and Hela cells infected with SeV for indicated times. (B) Western blot analysis of TRIM26 protein expression in mouse primary peritoneal macrophages stimulated with LPS or poly(I:C) for indicated times. (C) Western blot analysis of TRIM26 protein expression in mouse peritoneal macrophages treated with mIFN-β for indicated times (left). Western blot analysis of TRIM26 protein expression in mouse peritoneal macrophages transfected with STAT1 siRNA, STAT2 siRNA and control siRNA (right, upper panel), or pretreated with IFNR1 antibody and control antibody for 2 h (right, lower panel), then simulated with LPS for indicated times. (D) Fluorescent images of HEK293 cells transfected with GFP-TRIM26 and then stimulated with SeV (MOI 1), VSV (MOI 0.1) or IFN-β for 8 h. Nuclei were detected with DAPI (blue). (E) Western blot analysis of TRIM26 protein expression in nuclear and cytoplasmic fractions from Hela cells infected with SeV for 4 and 8 h. (F) Immunofluorescent images of mouse peritoneal macrophages stimulated with 100 ng/ml LPS for 10 h. Endogenous TRIM26 was analyzed by immunostaining with anti-TRIM26 antibody (red). Similar results were obtained from three independent experiments.