The effects of Honokiol on the cell viability and proportion of GBM8401 SP cells.

<p>(A) GBM8401 parental or SP cells were incubated with series concentrations of Honokiol for 48 h and the cell viability was examined by SRB assay. (B) GBM8401 cells were incubated with the absence or presence of specific inhibitor Verapamil (50 μM) and Honokiol (2.5 and 5 μM) for 48 h, respectively. After washed with PBS and centrifugation, SP assay was performed followed by staining the cells with Hoechst33342 (5 μg/ml) for 90 min.