Equivalent surface expression of CCR2 on MDSCs from DIO and NW mice.

<p>(A) DIO and NW mice were challenged as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118784#pone.0118784.g001" target="_blank">Fig. 1</a>. Tumor-bearing kidneys (TK) or tumor-free contralateral kidneys (CK) were harvested at the times indicated. Histograms show representative surface expression of CCR2 on Ly6C⁺ or Ly6G⁺ MDSC from DIO or NW mice. Open histograms = isotype control; shaded histograms = CCR2 staining. (B) Pooled data showing the % of MDSCs that express CCR2 in tumor-bearing kidneys (TK) and contralateral kidneys (CK). Gating on Ly6C⁺ or Ly6G⁺ MDSC shows strong and equivalent expression of CCR2 on MDSC subpopulations from DIO and NW mice. (C) Increased overall frequencies of CCR2⁺ MDSC from both Ly6C⁺ and Ly6G⁺ subsets when calculated as a percentage of total live cells within tumor-bearing kidneys. For B and C, n = 4–6 mice per group from two experiments. Bars indicate mean +/- sd. * = p < 0.05.