Required oxygen permeation rate in the PDMS membrane for chick embryo culturing.

<p>The permeating rates of 0.1-, 0.5-, and 1.00-mm-thick PDMS membranes were measured with an oxygen permeation analyzer, and the normalized results were 1150, 413, and 191 mL/day, respectively. In the current study, the lowest oxygen volume for embryo survival was estimated to be 286 mL/day (corresponding to a membrane thickness 0.55 mm), according to the total oxygen volume (6 L) consumed by a chick embryo during the embryonic period (21 days) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118624#pone.0118624.ref042" target="_blank">42</a>].