Mito-Tempo diminished adipocyte differentiation.

<p>(A) ROS levels in human MSCs on Day 0 and Day 2 of differentiation. Cells were treated with 500 nM of TPP or Mito-Tempo for 4 h prior to measurement on Day 2. (B) Mito-Tempo diminished lipid accumulation. Human MSCs were treated with 500 nM of Mito-Tempo starting on Day 2 of differentiation. Cells were fixed and stained with oil red O on Day 14. The oil red-O was extracted with isopropanol and absorbance was measured at 518 nm. (C, D) Gene expression of PPARγ and adiponectin was decreased in the presence of Mito-Tempo on Day 7 and Day 14 of differentiation.*<i>P</i> < 0.05.