ROS mediate adipocyte differentiation of MSCs <i>in vitro</i>.

<p>(A) Fold increase in ROS on Day 2/Day 0 of differentiation. “NAC” and “NAC+diff” groups were treated with 5 mM NAC for 4 h prior to ROS measurement on Day 2. The NAC treatment was administered daily and lasted to Day 14. (B) Fold increase in ROS on Day 7/Day 0 of differentiation. (C) Fold increase in ROS on Day 14/Day 0 of differentiation. (D, E) NAC diminished lipid accumulation. Cells were fixed and stained with oil red O on Day 14. The oil red O was extracted with isopropanol and absorbance was measured at 518 nm. (F) Gene expression of PPARγ and adiponectin was decreased in the presence of NAC at Day 7 and Day 14 of differentiation compared to control. *<i>P</i> < 0.05.