Cdc42 depletion reduces the long-term CFTR internalized fraction.

<p>The upper diagrams summarize the procedures followed. 48 h after cell transfection by siRNA, PM proteins were labelled and the cultures were submitted to additional 24 h incubation at 37°C. Purification occurred either directly (-), from 200 μg clarified lysates, or alternatively, after a MESNA-mediated stripping step (+), from 600 μg clarified lysates. After densitometric quantification of Western blot images (representative example in the bottom left panel), (+) to 3×(-) ratios were calculated. The Ctrl RNAi value was used to define 100% of long-term internalized fraction. In the bottom right panel, histograms express the relative CFTR internal fractions normalized to the Ctrl RNAi condition. Data represent means ± SEM of 3 independent experiments, each performed in duplicate. ***: p<0.001.