FeD Mice have Modulated Frequencies of Splenic NK Cells and Tregs Early During the Infection.

<p>Representative flow cytometric dot plots of NK cells (<b>a</b>) and the percentage of NK cells (<b>b</b>) on day 3 and day 7 post-infection. Representative flow cytometric dot plots of Tregs (<b>c</b>) and the percentage of Tregs after gating on CD4⁺ T cells (<b>d</b>) on day 3 and day 7 post-infection. The numbers shown on the dot plots indicate the mean percentage of cells inside the gate ± S.E.M. On day 3 post-infection, <i>n</i> = 6 for control mice and <i>n</i> = 5 for FeD mice, except for Tregs, where <i>n</i> = 5 for the control mice. On day 7 post-infection, <i>n</i> = 6 for control mice and <i>n</i> = 6 for FeD mice. FeD = iron dextran, PBS = control. Statistically significant differences, shown by asterisks (* <i>P</i> < 0.05 ** <i>P</i> < 0.01), were determined by unpaired Student’s t-test.