Analytical sensitivity of serially diluted overnight grown bacterial culture spiked in human feces after 4h of enrichment on 2% agarose gel electrophoresis.

<p><b>a) <i>S</i>. Typhi.</b> Lane M: 100bp DNA ladder (Promega), Lane 1–9: represents DNA extracted from human feces spiked with different concentration of <i>S</i>. Typhi after 4hrs of enrichment (1.5 X 10⁸ CFU/ml—1.5 X 10⁰ CFU/ml), Lane 10: DNA of unspiked human feces, lane 11: <i>S</i>. Typhi gDNA, Lane N: Negative control and <b>b) <i>S</i>. Paratyphi A.</b> Lane M: 100bp DNA ladder (Promega), Lane 1–9: represents DNA extracted from human feces spiked with different concentrations of <i>S</i>. Paratyphi A after 4hrs of enrichment (1.5 X 10⁸ CFU/ml—1.5 X 10⁰ CFU/ml), lane 10: DNA of unspiked human feces, lane 11: <i>S</i>. Paratyphi A gDNA, lane 12: <i>S</i>. Typhi gDNA. Lane N: Negative control.