Draxin-induced growth cone collapse is mediated through DCC.

<p>A. Growth cones of cortical neurons from newborn mice cultured for 60 h, pretreated for 1 h with function blocking anti-DCC antibodies (anti-DCC) or unrelated IgGs at the indicated concentrations followed by addition of draxin for 1 h as indicated, fixation and staining for F-actin. Scale bar = 10 μm. B. Quantification of growth cone collapse. For each experimental condition the growth cones of 40 neurons in each of 3 independent experiments were evaluated.