A, B, C, D, E: Expression of mRNA for COX-1, COX-2, TNF-α and CGRP in gastric mucosa.

<p>The expression of mRNA for COX-1, COX-2, TNF-α and CGRP was determined in intact rats and those pretreated with vehicle (saline) or H₂S-donor sodium hydrosulfide (NaHS) with and without combination with D, L- propargylglycine (PAG) and exposed 30 min later to 3.5 h of WRS (panel A). Semi-quantitative densitometry analysis of mRNA expression of COX-1, COX-2, TNF-α, CGRP transcripts normalized to β-actin mRNA expression (panel B). Results are mean ± S.E.M of four determinations. Significant change (p<0.05) as compared with the respective values in vehicle-control gastric mucosa was indicated by asterisk. Cross indicates a significant change (p<0.05) as compared to the value obtained in intact gastric mucosa. Asterisk and cross indicate a significant change (p<0.05) as compared to the values obtained in NaHS (5 mg/kg i.g.) alone pretreated group.