The Fur regulon is fully repressed upon MntS overproduction.

<p>Cells were grown in aerobic LB/arabinose medium with or without 0.5 mM MnCl₂ and aerated for 2.5 hr before harvesting. Data represent the mean of three independent cultures. Strains bearing <i>iucC’-lacZ</i> were JEM1369 (WT/pBAD24), JEM1370 (WT/pLW112), JEM1463 (Δ<i>fur</i> /pBAD24), and JEM1464 (Δ<i>fur</i>/pLW112). Strains bearing <i>fhuA’-lacZ</i> were JEM1395 (WT/pBAD24), JEM1396 (WT/pLW112), JEM1465 (Δ<i>fur</i> /pBAD24), and JEM1466 (Δ<i>fur</i>/pLW112). The pMntS (pLW112) plasmid encodes <i>mntS</i> driven by the <i>araBAD</i> promoter.