Structural mapping of TNAP M4 mutants.

<p>Degree of binding of TNAP and the indicated TNAP mutants to four epitope-mapped monoclonal anti-TNAP antibodies (of 19 studied). Binding was analyzed in the absence (black bars) and presence (white bars) of 1 mM CaCl₂. TNAP was bound to microtiter plates coated with AbM2 and bound TNAP (mutant) was detected with AbM2, recognizing the FLAG tag.