Examination of the interaction of RpaA, RpaB and ManR with Trx in <i>E</i>. <i>coli</i> cells.

<p>Expression levels of (<b>A</b>) RpaA, (<b>C</b>) RpaB, (<b>E</b>) ManR and Trx in the control <i>E</i>. <i>coli</i> Origami2 strain (Control), the strain expressing only TrxM_C35S (Trx), the strain expressing only a TF and the strain expressing both TrxM_C35S and a TF were examined. The whole cell extract (C), the soluble fraction (S) and the insoluble pellet fraction (P) were separated by 15% SDS-PAGE and stained with CBB. The interaction of (<b>B</b>) RpaA, (<b>D</b>) RpaB and (<b>F</b>) ManR with Trx was examined by non-reducing 12% SDS-PAGE and immunoblot analysis of soluble proteins from each strain. TFs and Trx were detected using a His-tag antibody and S-protein, respectively. ± indicates with or without 100 mM DTT treatment. Black arrow, white arrow and arrow head indicate the TF monomer, the Trx monomer, and the Trx-TF complex, respectively.