Examination of the interaction of Rre28, Rre37 and SphR with Trx in <i>E</i>. <i>coli</i> cells.

<p>Soluble proteins from the <i>E</i>. <i>coli</i> Origami2 strain (Control), the strain expressing only TrxM_C35S (Trx), the strain expressing only a TF and the strain expressing both TrxM_C35S and a TF were separated by non-reducing 12% SDS-PAGE and immunoblot analysis was performed. (<b>A</b>) Rre28, (<b>B</b>) Rre37 and (<b>C</b>) SphR were detected using a His-tag antibody (left panel) and Trx was detected using S-protein (right panel). ± indicates with or without 100 mM DTT treatment. Black and white arrows indicate the TF monomer and the Trx monomer, respectively.