Dopamine does not change the E-I balance in L5PyNs of 129/Sv and 5-HT_1AR-KO mice.

<p>(A) HPLC quantification of 5-HT and DA levels in tissue extracted from PFC of 129/Sv mice (White bar, n = 8, n = 4 animals) and 5-HT1AR-KO (Black bar, n = 8, n = 4 animals). Error bars represent S.E.M. (one-way ANOVA, *p<0.05, ** p< 0.01). (B) First row: Representative current responses in a L5PyN to layer 2/3 stimulation in PFC of a 129/Sv mouse (left column) and in presence of the D1R agonist SKF 81297 (10μM) (right column) recorded under voltage-clamp at various holding potentials (each response is the mean of 5 recordings). Vertical arrows indicate the stimulation onset. Second row: Corresponding conductance changes (gT, black line). Third row: Excitatory (gE, dark grey line) and inhibitory (gI, light grey line) conductance changes obtained from gT decomposition (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120286#sec002" target="_blank">Material and Methods</a>). (C) Representation of the E-I balance. The E-I balance does not change significantly in presence of the D1R agonist (SKF 81297, 10μM) or D1R antagonist (SCH 23390, 10μM) whatever the 129/Sv or 5-HT_1AR-KO mice strain.