Deglycosylation of recombinant proteins expressed in <i>P</i>. <i>pastoris</i>.

<p>(A). SDS-PAGE of the recombinant proteins expressed in <i>P</i>. <i>pastoris</i> with or without digestion of Endo H-P with post-fermentation method. M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 positive control(Horseradish Peroxidase) from the Glycoprotein Staining Kit;Lane 2 negative control(Soybean Trypsin Inhibitor) from Glycoprotein Staining Kit;Lane 3 mannosidase expressed in <i>P</i>. <i>pastoris</i>;Lane 4 deglycosylated mannosidase; Lane 5 phytase expressed in <i>P</i>. <i>pastoris</i>; Lane 6 deglycosylated phytase;Lane 7 <i>Dpn</i>I expressed in <i>P</i>. <i>pastoris</i>;Lane 8 deglycosylated <i>Dpn</i>I. (B). Glycoprotein staining of the recombinant proteins expressed in <i>P</i>. <i>pastoris</i> with or without Endo H-P digestion. All samples were loaded in the identical order with A.