Effects of Exogenously expressed CD45RO, RB, or RA-EGFP on phosphorylation of STAT3, MAPK, PKC and IκB.

<p>CD45^- U266 cells were transfected with untagged (mock) or CD45RO-EGFP, CD45RB-EGFP or CD45RA-EGFP expression plasmids, and either treated or untreated with IL-6 (10 ng/ml) at different time points. The representative blots are from three independent experiments and separate blotting using antibodies to P-STAT3, STAT3 (A), P-ERK, ERK (B), P-PKC, PKC (C) and P-IκB, IκB (D) are shown. The densities of protein bands were determined by densitometry and the data represent a change from the control mock density. * <i>p</i> < 0.05 vs mock control in the presence of IL-6; ** <i>p</i> < 0.01 vs mock control in the presence of IL-6 by a one-way ANOVA with HSD test.