Decreased B cells and increased myeloid cells in <i>Nfix</i> expressing cells in the periphery.

<p>C57Bl/6 mice were reconstituted with BM cells transduced with control MigR1 or <i>Nfix</i> vectors. <b>(A)</b> Graph of percentage number of GFP⁺ B cells (B220⁺, CD19⁺), myeloid cells (Gr-1⁺CD11b⁺), and <b>(C)</b> T cells (CD4⁺, CD8⁺) in the PB of MigR1 and <i>Nfix</i> chimeric animals 6 weeks post-transplant. <b>(B)</b> Representative flow cytometric analysis of PB cells in MigR1 and <i>Nfix</i> chimeric animals, 10 wk post-transplant, showing engraftment of GFP (left panel), B220⁺CD19⁺ B cells in the GFP^- and GFP⁺ fractions (middle panel, percentages given) and the Gr-1⁺CD11b⁺ myeloid cells in the GFP^- and GFP⁺ fractions (right panels, percentages given). Results are representative of 2 independent BMT experiments.