<i>Yersinia pestis</i> type III injectisome activates IL-1β and IL-18 cytokines early during lung infection.

<p><b>A.</b> IL-1β Western blot analysis performed on total lung homogenate harvested at 6, 12, and 24 hpi with LD₁₀₀ infection of <i>Y</i>. <i>pestis</i> strain CO92, compared to uninfected mice. <b>B.</b> IL-1β ELISA performed on total lung homogenates harvested at 6 and 24 hpi. <b>C.</b> Bacterial burdens from lungs of mice 24 hpi with 10⁴ CFU/ mouse with the strains mentioned (denoted by T3SS) or parent stain (unlabeled). <b>D.</b> IL-1β ELISA performed on total lung homogenates harvested at 24 hpi with with the strains mentioned. All infections were performed in triplicate, with representative analysis shown. * <i>p</i>< 0.05, ** <i>p</i><0.001.