<p>(A) Immunofluorescence analysis of claudins in co-culture of control MDCK II cells and claudin-2 knockout clone 1 (KO 1). Claudin-1, -3, -4, and -7 showed clearer and stronger signals at TJs in claudin-2 knockout cells than in control cells. Scale bar = 10 μm. (B) Quantification analysis of signal intensity of claudins at TJs in control MDCK II cells and claudin-2 knockout clones. The signal intensity of claudins at TJs in control cells and claudin-2 knockout clones was measured as described in <i>Materials and Methods</i>, and the relative signal intensity of each claudin was calculated as the ratio of the signal intensity in control cells (CTL) and claudin-2 knockout clones (KO 1 and 2) to the signal intensity in control cells. N = 4 f...
<p>Immunofluorescence microscopic analysis of claudin-1, -2, -3, -4 and -7 in control cells and ZO-1...
<p>(<b>A</b>) Histogram showing relative mRNA levels, determined by qRT-PCR, for the indicated trans...
<div><p>Tight junctions (TJs) regulate the movements of substances through the paracellular pathway,...
<p>(A) Immunofluorescence analysis of claudins and FLAG in co-culture of claudin-2 knockout cells an...
<p>(A) Immunoblots of claudin-2 and E-cadherin in control MDCK II cells and claudin-2 knockout clone...
<p>Immunofluorescence analysis of claudins and occludin in co-culture of control MDCK II cells and c...
<p>(A) Immunofluorescence analysis of claudin-1, -3, -4, -7, ZO-1, occludin, F-actin, and myosin hea...
<p>(A–E) Immunofluorescence analysis of claudins and ZO-1 or ZO-3 in MDCK II cells transfected with ...
<p>Immunofluorescence analysis of GFP, claudin-2 and ZO-1 in co-culture of control MDCK II cells and...
<p>(A) Effects of ZO-1 knockout on the localization of claudins. Control and ZO-1 knockout cells of ...
<p>(A) Immunofluorescence analysis of claudin-4 and occludin in claudin-2 knockout clone (CTL) and c...
<p>(A) Time course of TER and TER values at 6 days after seeding on filter inserts in control cells,...
<p>(A) TALEN binding sites in the claudin-2 gene. The left and right arms of TALEN targeting site ar...
<p>(A) Time course of TER and TER values at 6 days after seeding on filter inserts in control cells ...
<p>(A–C) Immunofluorescence analysis of ZO proteins in MDCK II cells transfected with TALEN construc...
<p>Immunofluorescence microscopic analysis of claudin-1, -2, -3, -4 and -7 in control cells and ZO-1...
<p>(<b>A</b>) Histogram showing relative mRNA levels, determined by qRT-PCR, for the indicated trans...
<div><p>Tight junctions (TJs) regulate the movements of substances through the paracellular pathway,...
<p>(A) Immunofluorescence analysis of claudins and FLAG in co-culture of claudin-2 knockout cells an...
<p>(A) Immunoblots of claudin-2 and E-cadherin in control MDCK II cells and claudin-2 knockout clone...
<p>Immunofluorescence analysis of claudins and occludin in co-culture of control MDCK II cells and c...
<p>(A) Immunofluorescence analysis of claudin-1, -3, -4, -7, ZO-1, occludin, F-actin, and myosin hea...
<p>(A–E) Immunofluorescence analysis of claudins and ZO-1 or ZO-3 in MDCK II cells transfected with ...
<p>Immunofluorescence analysis of GFP, claudin-2 and ZO-1 in co-culture of control MDCK II cells and...
<p>(A) Effects of ZO-1 knockout on the localization of claudins. Control and ZO-1 knockout cells of ...
<p>(A) Immunofluorescence analysis of claudin-4 and occludin in claudin-2 knockout clone (CTL) and c...
<p>(A) Time course of TER and TER values at 6 days after seeding on filter inserts in control cells,...
<p>(A) TALEN binding sites in the claudin-2 gene. The left and right arms of TALEN targeting site ar...
<p>(A) Time course of TER and TER values at 6 days after seeding on filter inserts in control cells ...
<p>(A–C) Immunofluorescence analysis of ZO proteins in MDCK II cells transfected with TALEN construc...
<p>Immunofluorescence microscopic analysis of claudin-1, -2, -3, -4 and -7 in control cells and ZO-1...
<p>(<b>A</b>) Histogram showing relative mRNA levels, determined by qRT-PCR, for the indicated trans...
<div><p>Tight junctions (TJs) regulate the movements of substances through the paracellular pathway,...