c-Myc acts on E-Boxes in the promoter of the miR-23b-27b cluster.

<p>A, Diagram representing the c-Myc binding sites in the miR-23b-27b cluster promoter region. B and C, ChIP assays were performed by IP with either anti-c-Myc antibody or control IgG. D, c-Myc regulates the promoter of the miR-23b-27b cluster. For the luciferase reporter assay, cells were co-transfected with empty vector or c-Myc plasmids and the luciferase reporter plasmid carrying promoter constructs containing site 1 or site 1 mutant (n = 3, unpaired t-test, ns, not significant,** P < 0.01).