Both <i>urp1</i>⁺ and <i>urp2</i>⁺ cells in the spinal cord are GABAergic neurons in adult zebrafish.

<p>Simultaneous expression of <i>urp1</i> and <i>urp2</i> revealed by double fluorescent ISH (TAMRA, red for <i>urp1</i> and Cy5, white for <i>urp2</i>) in adult spinal cord, coupled to a fluorescent immunostaining for GAD_65/67 (Alexa Fluor 488, green) <b>(A</b>, <b>B)</b>. Both <i>urp1</i>⁺ and <i>urp2</i>⁺ cells are GAD⁺<b>(A, B)</b>. Arrows designate triple-stained cells <b>(A, B)</b>. Note the occurrence of some doubly-positive cells (<i>urp2</i>/GAD) that do not contain any <i>urp1</i> (arrowhead) <b>(A)</b>. Asterisks designate GABAergic interneurons located at the dorsal part of the spinal cord. The white dash line indicates the central canal. <b>A</b>, sagittal section with dorsal up; <b>B</b>, coronal section with dorsal up. Scale bars: 15 μm.