Tpl2 promotes Th1, but not Th17, differentiation in a CD45RB T cell transfer model of colitis.

<p>Wild type or <i>Tpl2</i>^{<i>−/−</i>} naïve T cells (CD45RB^hiCD25^-CD4⁺) were injected i.p. into female Rag-deficient mice. (A) Weight loss curves as a percentage of original body weight. (B) Measure of serum cytokine levels in colitic mice. Significance was measured by one-tailed Student’s t-test. (C) Representative histologic images are shown along with scoring for total pathology in the colon. Pathology scores were evaluated using Mann-Whitney U test. (D) Proportions of IFNγ and IL-17A in the spleen and mesenteric lymph nodes as measured by intracellular staining and flow cytometry. Cells were gated on CD4⁺TCRβ⁺ cells. Significance was measured by one-tailed Student’s t-test. (E) Total cells and CD4⁺TCRβ⁺ cells in the spleen and mesenteric lymph nodes. (F) Total CD4⁺IFNγ⁺ and CD4⁺IL-17A⁺ cells in the spleen and mesenteric lymph nodes. N≥12. Pooled from 3 independent experiments. Error bars represent means ± sem. *p<0.05.