DHT mediates cell proliferation and Bicalutamide (Bic) abrogates this effect.

<p>(A) MDA-MB-453 cells treated with indicated treatments for 48 hours and their morphology was observed at higher magnification and randomly selected microscopic fields were photographed. (B) 3 x 10⁵ cells were plated in 35mm dish and the cell were treated with IC₅₀ concentrations of the various drugs and harvested at 48 hours for DNA content analysis by flow cytometry. <b>(C)</b> AR binding to the promoter of CDT and KLF by ChIP-qPCR showing the binding of AR to CDT promoter in presence of Paclitaxel and various other treatments.(D) ChIP-qPCR showing the binding of AR to KLF promoter in presence of Paclitaxel and various other treatments. The gel panel below the picture shows the standard PCR validation of the result. All the means and standard deviation are obtained from three independent experiments. Pac: Paclitaxel; Bic: Bicalutamide; Cyclo: Cyclophosphamide; Fluoro: 5'-Fluorouracil, M: Marker, 1: Input, 2: Untreated control, 3: DHT +Pac,: 4: DHT+ Bic +Pac, 5: Bic + Pac, 6: Bic.